Gene Construction Kit 3.0.4

Gene Construction Kit 3.0.4 is considered as an intuitive and effective utility that offers the easy graphic manipulation of DNA sequences and is  equipped with many sophisticated drawing capabilities. GCK allows customized formatting of any sequence text, feature, annotation, or graphic element.

In addition, GCK keeps track of segment history and offers the flexibility of displaying the same segment of DNA in a number of ways to emphasize key features on which the end-user wishes to focus. GCK was named the "Best Plasmid Mapping Software" by the Biotechnology Software and Internet Journal for four straight years.
GCK readily generates high quality black and white or color illustrations for journal submissions, slides, posters, or papers. It is ideal for designing and managing complex cloning projects or assembling DNA sequences for use in sequence analysis programs such as the Gene Inspector®.
Major Features:

1. Manipulate actual DNA sequences through a unique and innovative graphical interface
2. Ideal for planning and tracking complex construction projects
3. Identify PCR primers, and add them as features to a construct
4. View construct either as a text sequence or graphically, linear or circular
5. Identify and label introns in a DNA sequence - automatically skip them when translating the protein
6. Single search submission to query multiple online databases (KEGG, PubMed, PDB, GO, EntrezGene, etc.)
7. Automatically monitor and adjust DNA fragment ends during cutting and pasting
8. Custom formatting for nearly any graphic object - define font, color, size, line thickness, pattern, shape, direction, etc.
9. Search, download, and import GenBank and EMBL files with all feature table items translated automatically into GCK graphical elements
10. Perform automated shotgun cloning experiments - quickly generate a library of GCK constructs
11. Unique Chronography feature tracks the history of any given DNA segment and maintains alternative views
12. Cut and paste DNA segments from one construct to another - including segment history defined in chronography
13. View predicted electrophoresis patterns of restriction digests including partial digests
14. Create illustrations containing multiple constructs, gels, sequence listings, free text, legends and other graphics
15. Search Files module provides sophisticated database-like capabilities - save storage locations, project names, plasmid reference numbers, etc.
16. Create silent mutations to add or remove a restriction site from a sequence without changing the coding information
17. Mark restriction sites using one or more completely editable and expandable enzyme lists
18. Find Open Reading Frames using any codon table you specify
19. Frame segments of DNA in the sequence display - encompass site markers, sequence, or other regions of interest of a construct
20. Use 3-letter or 1-letter amino acid representation for protein sequences
21. Annotate sequence listings with custom 'adornments' to highlight sequence features
22. Display and manipulate multiple constructs in the same illustration window
23. Add arrows, rectangles, ellipses (filled or empty) and text to illustrations